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Irap

What is it?
irap is a syringe system which is used to produce autologous condition serum (ACS)

What is ACS?
ACS contains active quantities of autologous antiinflammatory and regenerative cytokines.

How does it work?
Whole blood is taken from the horse into the irap syringe. This is then incubated, centrifuged and the serum extracted for re-injection into the horse from which the blood was taken.

What can it be used for?
Joint lameness, muscle injuries, tendon and ligament injuries.

Has it been tested?
A blinded placebo controlled trial gave excellent results
The patented irap syringe
The patented irap syringe was developed in Germany by Orthogen GmbH . It contains specially treated glass spheres which encourage the growth of white blood cells which produce anti-inflammatory cytokines

Flowchart irap ACS proccessing
Blood Collection
Incubation of syringe
Centrifuge incubated syringe
  • Blood Collection withirap 50ml-syringe under routine aseptic conditions
  • 1-2 syringes are required per patient
  • Label syringe pateirnt data
  • Incubation of syringe at 37 C 24 kours
  • Process each patient's blood separately
  • Centrifuge incubated syringe 10 min at 3700 rpm
  • Load centrifuge symmetrically
Disinfect syringe
Aliquote autologous conditioned serum
Immediately label 5 ml - syringes
  • Disinfect syringe thoroughly
  • Carefully aspirate serum with 20 ml- syringe
  • Aliquote autologous conditioned serum through filter / adapter into 5 ml syringes
  • Close 5 ml - syringes with sterile caps
  • Immediately label 5 ml - syringes with patient data
  • Keep all syringes of one patient in a store package at 18 C until use
  • Shelf Life at least 7 months

Summary of Studies using ACS produced with the irap syringe

Frisbie, Kawcak and McIlwraith, Colorado State University. 16 horses in placebo controlled blinded study. Used experimentally induced OA model. Horses treated with ACS had significantly improved lameness in OA joints even at 5 weeks post treatment compared with placebo treated controls. (AEEP Proceedings 51 (2005) 374-375)

Meijer, Reinecke et al, Orthogen GmbH. Demonstrated that culturing blood in presence of specially treated glas spheres induces significant increase in anti-inflammatroy cytokines in whole (human) blood.


Cytokine T=0 hours T=0.5 hours T=24 hours Increase
IL-4 8.1 7.8 17.2 112%
IL-10 4.1 4.3 8.9 117%
IL-13 187 192 189 -
IL-1Ra 73 778 10254 13946%
IL-1ß <3.9 <3.9 <3.9 -
TNF-a <15.6 <15.6 <15.6 -
(Inflamm. Res. 52 (2003) 404-407)  

Irap has been used on thousands of horses and even more humans with excellent results.
Genitrix Ltd      Genitrix House      Daux Road      Billingshurst      West Sussex      RH14 9SJ
t: +44 (0) 1403 786345      f: +44 (0) 1403 786346      e: mail@genitrix.co.uk